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KMID : 1101420150470040243
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Korean Journal of Clinical Laboratory Science
2015 Volume.47 No. 4 p.243 ~ p.250
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Optimal Fixation and Decalcification Methods for Bone Marrow Biopsy
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Choi Myung-Sub
Lee Hyun-Sup
Kwon Hyuk-Chul
Bae Moon-Hwan
Ko Young-Hyeh
Kim Hee-Jin
Lee Beom-Se
Koo Bon-Kyung
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Abstract
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A bone marrow biopsy that has undergone decalcification with 10% nitric acid could not be used for various pathological tests and had extremely limited reproducibility. The fixing solution of each experimental group was differentiated in usage, one solution including acid and the other not. The use of the decalcification solution was separated into either acidic or alkaline (EDTA), and further experiments were conducted with differing time phases. When using a fixing solution and decalcification solution which included acid, the specimens were faulty to the extent that all pathological tests were impossible. However specimens that were processed with an EDTA type decalcification solution did not display a non-specific reaction in EBV ISH and were even able to produce results that were at a level suited to various studies or a pathological diagnosis in the FISH, DNA, RNA tests. By improving the fixing and decalcification of bone marrow biopsy, the study was able to make possible ISH, FISH, DNA tests as well as RNA study, and secured the sensitivity, specificity, and reproducibility of various test methods. The stabilization of various test methods that use bone marrow biopsy contributes to the diagnosis, prognosis, prediction, treatment of the patient and provide guidelines for decision-making.
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KEYWORD
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Bone marrow biopsy, Decalcification, Fixation, Methods
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